The Use of Atomic Force Microscopy for 3D Analysis of Nucleic Acid Hybridization on Microarrays
نویسندگان
چکیده
Oligonucleotide microarrays are considered today to be one of the most efficient methods of gene diagnostics. The capability of atomic force microscopy (AFM) to characterize the three-dimensional morphology of single molecules on a surface allows one to use it as an effective tool for the 3D analysis of a microarray for the detection of nucleic acids. The high resolution of AFM offers ways to decrease the detection threshold of target DNA and increase the signal-to-noise ratio. In this work, we suggest an approach to the evaluation of the results of hybridization of gold nanoparticle-labeled nucleic acids on silicon microarrays based on an AFM analysis of the surface both in air and in liquid which takes into account of their three-dimensional structure. We suggest a quantitative measure of the hybridization results which is based on the fraction of the surface area occupied by the nanoparticles.
منابع مشابه
An Efficient Covalent Coating on Glass Slides for Preparation of Optical Oligonucleotide Microarrays
Objective(s): Microarrays are potential analyzing tools for genomics and proteomics researches, which is in needed of suitable substrate for coating and also hybridization of biomolecules. Materials and Methods: In this research, a thin film of oxidized agarose was prepared on the glass slides which previously coated with poly-L-lysine (PLL). Some of the aldehyde groups of the activated aga...
متن کاملHybridization of polynucleotides on functionalized self-assembled monolayer
For the hybridization of single-stranded polynucleotides at a solid-liquid interface without any modifications of the polynucleotides, a self-assembled monolayer (SAM) containing anthryl groups which have an affinity for nucleic acid bases was prepared. The hybridization was investigated by surface plasmon resonance (SPR) and atomic force microscopy (AFM). Results of SPR measurement indicated t...
متن کاملImpact of surface chemistry and blocking strategies on DNA microarrays.
The surfaces and immobilization chemistries of DNA microarrays are the foundation for high quality gene expression data. Four surface modification chemistries, poly-L-lysine (PLL), 3-glycidoxypropyltrimethoxysilane (GPS), DAB-AM-poly(propyleminime hexadecaamine) dendrimer (DAB) and 3-aminopropyltrimethoxysilane (APS), were evaluated using cDNA and oligonucleotide sub-arrays. Two un-silanized gl...
متن کاملCombination of fluorescence in situ hybridization and scanning force microscopy for the ultrastructural characterization of defined chromatin regions
متن کامل
Ultra structural characteristics of methicillin resistant Staphylococcus aureus cell wall after affecting with lytic bacteriophages using atomic force microscopy
Objective(s): During the last years with increasing resistant bacteria to the most antibiotics bacteriophages are suggested as appropriate treatment option. To investigate lytic activity of bacteriophages there are indirect microbial procedures and direct methods. The present study to complement microbial procedures and investigate ultra-structural characteristics of infection bacterium-phage u...
متن کامل